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Vitamin B12 Test Also known as: Vitamin B12 (Cyanocobalamin) Test Price, Normal Range, Procedure, Result

Check Vitamin B12 Test Also known as: Vitamin B12 (Cyanocobalamin) Test Procedure, Result and Cost in India.

Frequently Asked Questions

  • To diagnose vitamin B12 deficiency, considering symptoms like fatigue, weakness, numbness, or memory problems.
  • To assess vitamin B12 absorption issues, even if dietary intake seems sufficient.
  • To identify the underlying cause of a suspected B12 deficiency, such as dietary habits or digestive problems.
  • To determine the need for further testing or treatment, like B12 supplements.
  • The test is important for pregnant women, elderly people and psychiatric inpatients with non-cognitive problems.

The Vitamin B12 Cyanocobalamin Test measures the level of vitamin B12 in your blood. Cyanocobalamin is a specific form of vitamin B12 commonly used in this test.

Vitamin b 12 test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.

  • High levels of Vitamin B12 may be seen in Kidney failure, Diabetes, Liver disease and with intake of Vitamin C/Vitamin A/Estrogens. 
  • Low levels of Vitamin B12 may be seen in Anemia, Hyperthyroidism, worm infestation, pregnancy, smoking, hemodialysis, intake of aspirin/ anticonvulsants/ colchicine/ ethanol/ contraceptive hormones and multiple myeloma

People with the following conditions are given vitamin B12:

  • Frail bones (osteoporosis)
  • Men with infertility
  • Infected tendons
  • Heart problems
  • Alzheimer’s illness
  • Intake problems
  • Bowel inflammation disease
  • Asthma
  • Cervical and other cancer prevention
  • Mental illness and sleep issues
  • Diabetes

There are no specific preparations required for the test. However, it is recommended that you consult with your healthcare provider before taking the test, as they may have specific instructions or recommendations based on your individual medical history and current health status.

The vitamin B12 test measures the amount of vitamin B12 in the blood, which is important for producing red blood cells, keeping the nervous system healthy, and creating DNA. It is crucial for your overall health and well-being. 

The vitamin B12 test is crucial for diagnosing and managing conditions related to vitamin B12 deficiency, including pernicious anemia, megaloblastic anemia, and neurological symptoms such as numbness and confusion. Early detection through this test helps prevent severe complications and supports better health outcomes for affected individuals, ensuring timely treatment. 

People with the following conditions are advised to undergo a vitamin B12 test: osteoporosis, male infertility, tendon infections, heart problems, Alzheimer’s disease, absorption issues, inflammatory bowel disease, asthma, cancer prevention, mental health concerns, sleep problems, and diabetes management. 

Individuals exhibiting symptoms of vitamin B12 deficiency, such as fatigue or weakness, as well as those with medical conditions affecting absorption, older adults, and vegans or strict vegetarians, should consider this test. Additionally, people taking certain medications which interfere with vitamin B12 absorption are also encouraged to get tested to ensure optimal health and prevent complications. 

Vitamin B12 deficiency is typically tested using a blood test that measures the level of vitamin B12 in the blood, sometimes along with additional tests like MMA and homocysteine level

The benefits include early detection of vitamin B12 deficiency, diagnosis of underlying conditions, and guidance for treatment like supplements or injections. 

Fasting is generally not required for a vitamin B12 test. However, if the test is part of a larger blood panel that requires fasting, it’s important to follow your healthcare provider’s instructions for accurate results. 

A blood test measuring vitamin B12 levels (such as the vitamin B12 test) directly indicates whether there is a deficiency. This assessment is often supported by an elevate level of methylmalonic acid (MMA) and homocysteine, which can further confirm the deficiency. 

Yes, vitamin B12 is crucial during pregnancy for healthy growth and development of the foetus. This is particularly important for women following a vegan or vegetarian diet, as they may require additional supplementation to meet their nutritional needs and support their baby’s health. 

To confirm a vitamin B12 deficiency, several tests are done. These include checking for low levels of vitamin B12 in the blood and elevated levels of methylmalonic acid (MMA) and homocysteine. An intrinsic factor antibody test may also be performed to look for possible autoimmune causes of the deficiency. 

Vitamin B12 levels can be raised through making dietary changes, such as increased intake of animal products or fortified foods. Additionally, taking oral supplements or receiving injections can help, especially if the body has trouble absorbing B12 from food effectively. 

A low vitamin B12 level can lead to megaloblastic anaemia, neurological symptoms such as neuropathy, weakness, confusion, and dementia, and other health issues like fatigue and shortness of breath.

Normal vitamin B12 levels range from 200 to 900 pg/mL, varying slightly by laboratory testing.

Blood tests for measuring vitamin B12 levels are typically conducted in a clinical setting. However, some laboratories, such as HospitalandDoctors Partner Healthcare, provide convenient home sample collection services, allowing patients to have their blood drawn in the comfort of their own homes. 

Test results typically take a few days to a week after the blood sample is collected, depending on the laboratory’s processing time. 

If you follow a vegetarian diet, especially a vegan diet that excludes all animal products, you may be at a higher risk of vitamin B12 deficiency. While vegetarians who consume dairy and eggs may still get some vitamin B12 from these sources, vegans are more likely to have inadequate intake since vitamin B12 is primarily found in animal-derived foods. As a result, your healthcare provider may recommend a vitamin B12 test, particularly if you have been following a vegetarian or vegan diet for an extended period or if you show any symptoms of deficiency.
If your vitamin B12 blood test results indicate a deficiency, your healthcare provider will work with you to develop a treatment plan. This may include dietary changes and taking supplements and intramuscular vitamin B12 injections, especially if you have severe deficiency or absorption issues.
If you are diagnosed with vitamin B12 deficiency, your healthcare provider may recommend additional tests to determine the underlying cause. These may include the folate (vitamin B9) test, as folate deficiency can cause similar symptoms to vitamin B12 deficiency. You could also be asked to take the intrinsic factor antibody test that helps diagnose pernicious anaemia or the homocysteine and methylmalonic acid tests that help identify vitamin B12 deficiency. Your healthcare provider may also recommend tests for conditions like celiac disease or atrophic gastritis, which can interfere with vitamin B12 absorption. When discussing a treatment plan, it’s essential to enquire about the vitamin B12 test price or the vitamin B12 test cost to plan your expenses accordingly.

Vitamin B12 (Cyanocobalamin) Test Overview

Vitamin B12 is crucial for healthy red blood cells, brain function, energy production, and nerves. We can’t make it ourselves and vegetarians are especially at risk for deficiency since it’s found mainly in animal products. A vitamin B12 test measures the amount of this essential vitamin in your blood. This test helps doctors diagnose a B12 deficiency caused by dietary factors or absorption problems and determine if further tests or treatments like B12 supplements are needed.

Reasons for Undergoing the Vitamin B12 Test 

There are multiple reasons a healthcare provider may order a vitamin B12 test, including diagnosis, monitoring, and evaluation of: 

  • Diagnosing suspected vitamin B12 deficiency based on symptoms such as fatigue, weakness, loss of balance, numbness, pain, or tingling in the arms and legs. 

  • Assessing pernicious anemia, a condition that occurs when the body lacks intrinsic factors necessary for vitamin B12 absorption. 

  • Investigating megaloblastic anemia if blood tests show abnormal results. 

  • Monitoring the effects of medications that may interfere vitamin B12 absorption, such as proton-pump inhibitors or histamine blockers. 

  • Determining the risk of deficiency in individuals, especially those on a vegan or strict vegetarian diet. 

  • Exploring neurological symptoms like neuropathy, confusion, or dementia that could be linked to vitamin B12 deficiency. 

  • Assessing overall nutritional status and ensuring that patients maintain adequate vitamin levels to prevent potential health complications related to deficiencies. 

How can I book my test?

Booking a vitamin B12 test is a straightforward process. The first step is to consult with your healthcare provider, who will assess your symptoms and medical history to determine if the test is necessary. If they recommend the test, they will provide you with a referral or directly order the test for you.

HospitalandDoctors Partner Healthcare, offer convenient online booking services along with the option of home sample collection, where a qualified technician will visit your home to collect the blood sample for the vitamin B12 blood test. When booking your test, it’s essential to enquire about the vitamin B12 test price to plan your expenses accordingly.

Where can I see or get my test results?

After completing your vitamin B12 blood test, you can typically access your test results through HospitalandDoctors Partner Healthcare’s online portal. You may also receive your reports via email or SMS, allowing you to download them for your reference or share them with your healthcare provider.

Your healthcare provider will discuss the test results with you, explaining what they mean and recommending any necessary follow-up actions. They will help you understand if your vitamin B12 levels are within the normal range or if there are any concerns that require further investigation or treatment.

Symptoms that call for vitamin B12 test

Several symptoms may indicate a need for a vitamin B12 test. These include:

  • Fatigue and weakness
  • Shortness of breath
  • Numbness or tingling in hands and feet
  • Digestive issues like diarrhoea or constipation
  • Mood changes or depression
  • Memory problems or difficulty concentrating

If you experience any of these symptoms, it’s essential to consult your healthcare provider. They may recommend a vitamin B12 test to determine if a deficiency is the underlying cause.

Other factors that may prompt your doctor to suggest a vitamin B12 test include following a strict vegetarian or vegan diet, having a history of gastrointestinal disorders like celiac disease or Crohn’s disease, or having a family history of pernicious anaemia or other autoimmune conditions.

What does the vitamin B12 test detect?

The vitamin B12 test is performed using the Electrochemiluminescence Immunoassay (ECLIA) method. It measures the level of vitamin B12 in your blood and helps detect whether you have a deficiency, normal levels, or elevated levels of this essential nutrient.

Low levels of vitamin B12 may indicate malabsorption issues, dietary deficiencies, or other underlying health conditions. Malabsorption can occur due to factors like intestinal disorders, surgeries affecting the digestive tract, or the use of certain medications that interfere with vitamin B12 absorption.


On the other hand, high levels of vitamin B12 can sometimes indicate liver disease, leukaemia, or other conditions. Your healthcare provider will interpret your vitamin B12 blood test results in the context of your overall health and medical history.

When can a doctor recommend the vitamin B12 test?

Your doctor may recommend a vitamin B12 test in several situations:

  • If you show symptoms of vitamin B12 deficiency, such as fatigue, weakness, numbness, or tingling sensations in your hands and feet.
  • If you have a history of gastrointestinal disorders that can affect vitamin B12 absorption, such as celiac disease, Crohn’s disease, or atrophic gastritis.
  • If you follow a strict vegetarian or vegan diet, you may be at a higher risk of vitamin B12 deficiency since this nutrient is primarily found in animal-derived foods.
  • If you have neurological symptoms like memory problems, difficulty concentrating, or balance issues, which can be associated with vitamin B12 deficiency.
  • If you have a family history of pernicious anaemia or other autoimmune conditions that can impact vitamin B12 absorption.

Preparations required for a vitamin B12 test

The good news is that the preparations required for a vitamin B12 test are minimal. Unlike some other blood tests, you do not need to fast or make any significant dietary changes before the test.

The good news is that the preparations required for a vitamin B12 test are minimal. Unlike some other blood tests, you do not need to fast or make any significant dietary changes before the test.
However, it’s essential to inform your healthcare provider about any medications or supplements you are currently taking. Some medications may interfere with the test results or affect your vitamin B12 levels. Your doctor will guide you on whether you need to temporarily stop any medications before the test.
If you are taking high doses of biotin (>5 mg/day), it is advised to stop at least 8 hours before the test as it may interfere with test results.

Procedure of a vitamin B12 blood test

The procedure for a vitamin B12 blood test is relatively simple and involves a standard blood draw. Here’s what you can expect:

  • A healthcare professional, such as a phlebotomist or nurse, will ask you to roll up your sleeve and expose the arm from which they will draw the blood sample.
  • They will then apply a tourniquet to your upper arm to make the veins more prominent and easier to locate.
  • Using a sterile needle, they will carefully insert it into the vein and collect a small blood sample into a test tube.
  • Once the required amount of blood is collected, they will remove the needle and apply pressure to the site with a cotton ball or gauze to stop any bleeding.
  • They will then place a bandage over the site to keep it clean and protected.

The entire procedure usually takes just a few minutes, and most people experience only minor discomfort during the blood draw. The collected blood sample is then sent to a laboratory for analysis to determine your vitamin B12 levels. When booking your test, it’s essential to enquire about the vitamin B12 test cost to plan your expenses accordingly.

Risks associated with vitamin B12 test

The risks associated with a vitamin B12 test are minimal, as it is a routine and generally safe procedure. However, as with any blood test, there are a few potential side effects to be aware of:

  • Pain or bruising at the injection site: This is usually minor and resolves on its own within a few days.
  • Infection: There is a very small risk of infection at the site where the needle was inserted. This is rare, as healthcare professionals use sterile techniques and disposable needles to minimise the risk of infection.
  • Feeling lightheaded or dizzy: This is more likely to occur if you have a fear of needles or a history of fainting during medical procedures. If you experience these symptoms, inform the healthcare professional so they can help you lie down and provide necessary care.

Vitamin B12 Normal Test Range

The normal range for vitamin B12 levels can vary slightly depending on the laboratory and the specific testing method used. However, the general guidelines for interpreting vitamin B12 test results are as follows:

Vitamin B12 Level (pg/mL)

Interpretation

Below 200

Low, may indicate deficiency

200 to 300

Borderline, may require further testing

300 to 800

Normal range

Above 800

High, may indicate liver disease or other conditions

If your vitamin B12 test results fall within the normal range, it generally means that your body has sufficient levels of this nutrient. However, if your levels are low or borderline, your healthcare provider may recommend further testing or treatment to address any potential deficiencies.

List of Parameters Considered During the Vitamin B12 Test 

The vitamin B12 blood test is often used to measure parameters such as: 

  • The amount of vitamin B12 in the blood is measured, with normal levels usually ranging from 200 to 900 pg/mL.  

  • To aid in diagnosing vitamin B12 deficiency, especially in the early stages when B12 levels might still be normal, additional tests such as methylmalonic acid (MMA) and homocysteine tests may be requested. 

Home Collection for Vitamin B12 Test Near You 

HospitalandDoctors Partner Healthcare offers a convenient home sample collection service for the vitamin B12 test, delivering comfort and safety right to your doorstep. A trained phlebotomist will visit your home to collect blood samples, ensuring the highest standards of handling and accuracy. By opting for a home sample collection service, you can skip the hassle of visiting a diagnostic centre and conveniently obtain timely, reliable results. 

This service makes it easy for you to manage your health from the comfort of your home. HospitalandDoctors Partner Healthcare is dedicated to making quality healthcare accessible for everyone. Our diagnostic solutions are designed to enhance your healthcare experience by focusing on your needs and preferences, ensuring you receive excellent care without any added stress or inconvenience. 

Vitamin B12 (Cyanocobalamin) Test Price

₹ 1,250

Disclaimer: This site does not replace professional medical advice for Vitamin B12 Test Also known as: Vitamin B12 (Cyanocobalamin) Test. Always consult your healthcare provider for concerns about your health.

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